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Etomidate is an ideal induction agent for thesecases, as it minimally affects HR and blood pressure (BP). Nevertheless, ?rst stable integrant harboringRep protein emerged which were under an inducible promoter capable of assem-bling viral particles upon providing Cap and transgene cassette in trans [ 97 ].Subsequently producer cell lines [ 99], containing all the needed AAV genes andpackaging cell lines [ 100 – 102] containing rep and cap genes were reported (Fig

Nevertheless, ?rst stable integrant harboringRep protein emerged which were under an inducible promoter capable of assem-bling viral particles upon providing Cap and transgene cassette in trans [ 97 ].Subsequently producer cell lines [ 99], containing all the needed AAV genes andpackaging cell lines [ 100 – 102] containing rep and cap genes were reported (Fig.